CRISPR/Cas-based Nanobiosesning for advanced virus detection
Viral diseases have received immense attention in the medical and healthcare fields because of the high transmission rates and difficulty in curing, including the recent COVID-19 issue. Nucleic acids are one of the attractive biomarkers to diagnose various viral-associated diseases precisely. Recently, the target nucleic acid-dependent trans-activating phenomenon of clustered regularly interspaced short palindromic repeats (CRISPR)/Cas has shown huge potential for developing sensitive and selective biosensors to detect targeted nucleic acids. However, the nucleic acid amplification steps are conventionally required for sensitive and selective monitoring of the target nucleic acid, requiring multistep reactions, expensive reagents, well-trained personnel, and sophisticated instrumentation.