BEGIN:VCALENDAR VERSION:2.0 PRODID:-//jEvents 2.0 for Joomla//EN CALSCALE:GREGORIAN METHOD:PUBLISH BEGIN:VEVENT UID:dbd4d92d932180e28534827393a97920 CATEGORIES:Colloquium CREATED:20220119T155926 SUMMARY:Professor Ming Hammond, University of Utah DESCRIPTION: RNA-based fluorescent biosensors to illuminate the dynamics and function o f chemical signals\nThe current decade has seen several unexpected discover ies of nucleotide-based signals that control the autonomous behavior of bac teria and immune cells. The newfound signaling pathways may provide new way s to combat antibiotic-resistant pathogens and to enhance the effectiveness of cancer immunotherapy treatments. However, critical questions remain abo ut the biochemistry of the signaling enzymes and cell biology of the signal s themselves. Tackling these questions requires tracking nucleotide-based s ignaling molecules in the complex environment of the cell, which poses a di fficult molecular recognition challenge.\nMy lab has taken a structure-base d design coupled to high-throughput screening approach and was among the fi rst to develop RNA-based fluorescent biosensors, or RBF biosensors, for liv e cell imaging. Our biosensors exhibit remarkable specificity and affinity for nucleotide-based signals, are the brightest to date in live cell imagin g studies, and can be rationally reprogrammed to sense new ligands. In this talk, I will present the design principles that enable effective allosteri c coupling of ligand binding to fluorescence activation of a small molecule chromophore. We have demonstrated performing, in essence, in vivo biochemi stry experiments to track dynamic effects of endogenous chemical cues and i nhibitor compounds on enzyme activity in live bacteria. This approach has r evealed a new strategy to combat antibiotic resistance. We also have applie d these biosensors to make several biological discoveries, including a sign aling pathway that regulates how some bacteria interact with redox reactive surfaces. Finally, I will describe another broad application for these bio sensors, as novel high-throughput screening assays for enzyme discovery.\nH osted by Professor Enver Izgu\nFor Zoom meeting information, please contact Loretta Lupo @ (mailto:lal275@chem.rutgers.edu)This email address is bein g protected from spambots. You need JavaScript enabled to view it.\n X-ALT-DESC;FMTTYPE=text/html:
RNA-based fluorescent biosensors to ill uminate the dynamics and function of chemical signals
The current decade has seen several unexpected disco veries of nucleotide-based signals that control the autonomous behavior of bacteria and immune cells. The newfound signaling pathways may provide new ways to combat antibiotic-resistant pathogens and to enhance the effectiven ess of cancer immunotherapy treatments. However, critical questions remain about the biochemistry of the signaling enzymes and cell biology of the sig nals themselves. Tackling these questions requires tracking nucleotide-base d signaling molecules in the complex environment of the cell, which poses a difficult molecular recognition challenge.
My lab has taken a structure-based design coupled to high-throughput sc reening approach and was among the first to develop RNA-based fluorescent b iosensors, or RBF biosensors, for live cell imaging. Our biosensors exhibit remarkable specificity and affinity for nucleotide-based signals, are the brightest to date in live cell imaging studies, and can be rationally repro grammed to sense new ligands. In this talk, I will present the design princ iples that enable effective allosteric coupling of ligand binding to fluore scence activation of a small molecule chromophore. We have demonstrated per forming, in essence, in vivo biochemistry experiments to track dynamic effe cts of endogenous chemical cues and inhibitor compounds on enzyme activity in live bacteria. This approach has revealed a new strategy to combat antib iotic resistance. We also have applied these biosensors to make several bio logical discoveries, including a signaling pathway that regulates how some bacteria interact with redox reactive surfaces. Finally, I will describe an other broad application for these biosensors, as novel high-throughput scre ening assays for enzyme discovery.
Hosted by Professor Enver Izgu
For Zoom meeting information, please contact Loretta Lupo @