BEGIN:VCALENDAR VERSION:2.0 PRODID:-//jEvents 2.0 for Joomla//EN CALSCALE:GREGORIAN METHOD:PUBLISH BEGIN:VEVENT UID:da0be3b68a1529181f62784bf8fa76af CATEGORIES:Colloquium CREATED:20201217T134908 SUMMARY:Wendy Zhong, Merck DESCRIPTION:
Label Free Molecular Imaging by Hi gh Resolution Mass Spectrometry—It’s Application in Pharmaceutical Industry
Matrix-assisted Laser Desorpti on Ionization mass spectrometry imaging (MALDI-MSI) has been widely applied in direct tissue analyses to characterize the biological distribution of a wide variety of molecules including small molecule metabolites, lipids, pe ptides and proteins. MSI is increasingly being used throughout the pharmace utical industry owing to its molecular specificity over conventional imagin g modalities such as quantitative whole-body autoradiography and immunohist ochemistry, which cannot differentiate parent drug versus metabolites. Thes e advantages of MALDI-MSI also make it a promising analytical tool for the analysis of a wide range of drug formulation-based applications by simultan eous monitoring of formulation uniformity, chemical composition, contaminat ion, degradation, and drug release.
In this presentation, I will discuss several unique/innovative approaches we developed and applied to solve challenge problems during drug discovery and development. Fatty liver disease phenotype is characterized by b oth an increase in the concentration and synthesis rate of neutral lipids ( NL) across the liver. The spatial distribution of multiple lipids classes i s poorly characterized in fatty liver disease. A novel method was developme nt to enhance ionization of neutral lipid via Sodium-Doped Gold-Assisted La ser Desorption Ionization. This method can allow investigators to obt ain spatial resolution of lipogenic flux by coupling imaging mass spectrome try and isotope tracers. Long-acting injectable (LAI) implants can deliver a drug over the course of several weeks to years; reducing the dosing frequency and improving patient adherence. An innovative appro ach of using MALDI-MSI to characterize the drug release process from LAI im plants was developed. This method provides definitive molecular level information about the chemical composition as well as the distribution of the APIs simultaneously. This is crucial information for a better understan ding of the underlying drug release mechanisms and the effect of formulatio n on drug distribution uniformity within the LAP and on release kinetics. p>
Webex Meeting li
nk: https://rutgers.webex.com/rutgers/j.php?MTID=m14
d55398e212d7e1640b3ef59eac227a
Meeting number: 120 443 2322
P
assword: RMg3KJpMF26
Join by
video system:
Dial
Join by phone:
+1-650-429-3300 USA Toll
Access code: 120 443 2322
& nbsp;
Hosted by Professor Gene Hall
X-ALT-DESC;FMTTYPE=text/html:Label Free Molecular Imaging by High Resolution Mass Spectrometry—It’s Applicatio n in Pharmaceutical Industry
Ma trix-assisted Laser Desorption Ionization mass spectrometry imaging (MALDI- MSI) has been widely applied in direct tissue analyses to characterize the biological distribution of a wide variety of molecules including small mole cule metabolites, lipids, peptides and proteins. MSI is increasingly being used throughout the pharmaceutical industry owing to its molecular specific ity over conventional imaging modalities such as quantitative whole-body au toradiography and immunohistochemistry, which cannot differentiate parent d rug versus metabolites. These advantages of MALDI-MSI also make it a promis ing analytical tool for the analysis of a wide range of drug formulation-ba sed applications by simultaneous monitoring of formulation uniformity, chem ical composition, contamination, degradation, and drug release.
In this presentation, I will discuss several unique/innovative approaches we developed and applied to solve challenge pr oblems during drug discovery and development. Fatty liver disease phe notype is characterized by both an increase in the concentration and synthe sis rate of neutral lipids (NL) across the liver. The spatial distribution of multiple lipids classes is poorly characterized in fatty liver disease. A novel method was development to enhance ionization of neutral lipid via S odium-Doped Gold-Assisted Laser Desorption Ionization. This method ca n allow investigators to obtain spatial resolution of lipogenic flux by cou pling imaging mass spectrometry and isotope tracers. Long-actin g injectable (LAI) implants can deliver a drug over the course of several w eeks to years; reducing the dosing frequency and improving patient adherenc e. An innovative approach of using MALDI-MSI to characterize the drug release process from LAI implants was developed. This method provide s definitive molecular level information about the chemical composition as well as the distribution of the APIs simultaneously. This is crucial inform ation for a better understanding of the underlying drug release mechanisms and the effect of formulation on drug distribution uniformity within the LA P and on release kinetics.
Webex Meeting link: https://rutgers.webe
x.com/rutgers/j.php?MTID=m14d55398e212d7e1640b3ef59eac227a
Meeting
number: 120 443 2322
Password: RMg3KJpMF26
Join by video system:
Dial
Join by phone:
+1-650-429-3300 USA Toll
Acces
s code: 120 443 2322
Hosted by Professor Gene Hall
DTSTAMP:20240328T144129 DTSTART:20210216T160000 DTEND:20210216T170000 SEQUENCE:0 TRANSP:OPAQUE END:VEVENT END:VCALENDAR