BEGIN:VCALENDAR VERSION:2.0 PRODID:-//jEvents 2.0 for Joomla//EN CALSCALE:GREGORIAN METHOD:PUBLISH BEGIN:VEVENT UID:0676580cad0b28aaeb939b8d68af3390 CATEGORIES:Colloquium CREATED:20201217T134508 SUMMARY:Siyuan Wang, Yale University DESCRIPTION: Image-based spatial genomics and multi-omics\nThe three-dimensional (3D) o rganization of the genome affects many genomic functions. The genome is hie rarchically organized into multiple 3D architectures including chromatin lo ops, domains, compartments, and regions associated with nuclear lamina and nucleoli. Changes in these architectures have been associated with normal d evelopment, aging, and a wide range of diseases. Despite its critical impor tance, understanding how the genome is spatially organized in single cells, how the organization varies in different cell types in mammalian tissue, a nd how the organization affects gene expression remains a major challenge f or the scientific and biomedical community. Previous approaches are limited by a lack of capacity to directly trace chromatin folding in 3D, and to si multaneously measure genomic organization in relation to other nuclear comp onents and gene expression in the same, single cells. To address these need s, we developed an image-based 3D genomics technique termed chromatin traci ng, which enables direct 3D tracing of chromatin folding along individual c hromosomes in single cells. Recently, we further developed Multiplexed Imag ing of Nucleome Architectures (MINA), which enables simultaneous measuremen ts of multiscale chromatin folding, associations of genomic regions with nu clear lamina and nucleoli, and copy numbers of numerous RNA species in the same, single cells in mammalian tissue. We are applying these developments to multiple lines of research on 3D genomics by depicting multiscale genomi c architectures associated with gene expression and other genomic functions in different types of cells and tissue undergoing different biological pro cesses. \nZoom Meeting Link: https://rutgers.zoom.us/j/96956706663?pwd=UENn NGdnRnhvcE5RUS9nWnMrMzN5Zz09&from=addon\nMeeting ID: 969 5670 6663\nPas sword: 477159\n\nJoin by Telephone:\nUS: +1 301 715 8592 or +1 312 626 6799 or +1 646 558 8656 or +1 253 215 8782 or +1 346 248 7799 or +1 669 900 912 8\nMeeting ID: 969 5670 6663\n\nInternational numbers:\nSkype for Business (Lync)\nhttps://rutgers .zoom.us/skype/94925703570 \n \nHosted by Professor Jason Zhang\n X-ALT-DESC;FMTTYPE=text/html:
Image-based spatial genomics and multi-omics
The three-dimensional (3D) organizat ion of the genome affects many genomic functions. The genome is hierarchica lly organized into multiple 3D architectures including chromatin loops, dom ains, compartments, and regions associated with nuclear lamina and nucleoli . Changes in these architectures have been associated with normal developme nt, aging, and a wide range of diseases. Despite its critical importance, u nderstanding how the genome is spatially organized in single cells, how the organization varies in different cell types in mammalian tissue, and how t he organization affects gene expression remains a major challenge for the s cientific and biomedical community. Previous approaches are limited by a la ck of capacity to directly trace chromatin folding in 3D, and to simultaneo usly measure genomic organization in relation to other nuclear components a nd gene expression in the same, single cells. To address these needs, we de veloped an image-based 3D genomics technique termed chromatin tracing, whic h enables direct 3D tracing of chromatin folding along individual chromosom es in single cells. Recently, we further developed Multiplexed Imaging of N ucleome Architectures (MINA), which enables simultaneous measurements of mu ltiscale chromatin folding, associations of genomic regions with nuclear la mina and nucleoli, and copy numbers of numerous RNA species in the same, si ngle cells in mammalian tissue. We are applying these developments to multi ple lines of research on 3D genomics by depicting multiscale genomic archit ectures associated with gene expression and other genomic functions in diff erent types of cells and tissue undergoing different biological processes.& nbsp;
Zoom Meeting Link: ht
tps://rutgers.zoom.us/j/96956706663?pwd=UENnNGdnRnhvcE5RUS9nWnMrMzN5Zz09&am
p;from=addon
Meeting ID: 969 5670 6663
Join by Telephone:
US: +1 301 715 8592 or +1 312 626 6799 or +1 646 558
8656 or +1 253 215 8782 or +1 346 248 7799 or +1 669 900 9128
Meeting ID: 969 5670 6663
International number
s:
Skype for Business (Lync)
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;
Hosted by Professor Jason Zhang
DTSTAMP:20240328T232007 DTSTART:20210126T160000 DTEND:20210126T170000 SEQUENCE:0 TRANSP:OPAQUE END:VEVENT END:VCALENDAR