BEGIN:VCALENDAR VERSION:2.0 PRODID:-//jEvents 2.0 for Joomla//EN CALSCALE:GREGORIAN METHOD:PUBLISH BEGIN:VEVENT UID:34ea9c0fce032974238387d8f64adfc5 CATEGORIES:Physical Chemistry Seminar CREATED:20170908T125059 SUMMARY:Physical Chemistry Seminar: Dr. Jing Yan DESCRIPTION:Dr. Jing YanDepartment of Molecular Biology, Princeton UniversityFriday, Se ptember 8, 2017\n12:00PM, Proteomics 120\n"Biophysical Principles of Biofil m Formation"\nBiofilms are surface-associated bacterial communities embedde d in an extracellular\nmatrix. Biofilm cells are more resistant to antibiot ics than their planktonic counterparts,\nwhich is a major problem in the co ntext of chronic infections. Investigations so far have\nfocused on the gen etic and regulatory features driving biofilm formation. However, still\nlac king is a fundamental biophysical understanding of how bacteria, in time an d space,\nbuild these three-dimensional structures that attach to surfaces and resist mechanical and\nchemical perturbations.\nIn this talk, I will pr esent our recent progresses in understanding\nthe biophysical principles of biofilm formation using Vibrio cholerae (the causal agent of\ncholera) as the model organism. We developed the first technology capable of imaging\nt hree-dimensional, living, growing biofilms with single-cell resolution. By combining the\nsingle-cell imaging technology with molecular genetics and b iochemistry, we discovered\nhow bacteria build biofilms cell by cell and ho w the biofilm architecture is influenced by\nspecific matrix components and by external stimuli including osmotic pressure differences\nand fluctuatio ns in nutrients. We also established platforms to measure biofilm material\ nproperties such as shear modulus and surface adhesion strength, and based on these\nmechanical data, we developed a new biofilm removal and transfer technology. Our\nultimate goal is provide a comprehensive understanding of how bacteria, given their\nlimited material choices and energy constraints, cooperate in space and time to synthesize\na unique, living soft material: the biofilm.\n X-ALT-DESC;FMTTYPE=text/html:
Friday, September 8, 2017< /p>
12:00PM, Proteomics 120
"Bi ophysical Principles of Biofilm Formation"
Biofilms are surface-assoc
iated bacterial communities embedded in an extracellular
matrix. Biofi
lm cells are more resistant to antibiotics than their planktonic counterpar
ts,
which is a major problem in the context of chronic infections. Inv
estigations so far have
focused on the genetic and regulatory features
driving biofilm formation. However, still
lacking is a fundamental bi
ophysical understanding of how bacteria, in time and space,
build thes
e three-dimensional structures that attach to surfaces and resist mechanica
l and
chemical perturbations.
In this talk, I will present our r
ecent progresses in understanding
the biophysical principles of biofil
m formation using Vibrio cholerae (the causal agent of
cholera) as the
model organism. We developed the first technology capable of imaging
three-dimensional, living, growing biofilms with single-cell resolution. By
combining the
single-cell imaging technology with molecular genetics
and biochemistry, we discovered
how bacteria build biofilms cell by ce
ll and how the biofilm architecture is influenced by
specific matrix c
omponents and by external stimuli including osmotic pressure differences
and fluctuations in nutrients. We also established platforms to measure
biofilm material
properties such as shear modulus and surface adhesion
strength, and based on these
mechanical data, we developed a new biof
ilm removal and transfer technology. Our
ultimate goal is provide a co
mprehensive understanding of how bacteria, given their
limited materia
l choices and energy constraints, cooperate in space and time to synthesize
a unique, living soft material: the biofilm.