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Professor Luke Lee, Harvard Medical School
Tuesday, February 20, 2024, 11:00am
 

Luke LeeNanomedicine via SANDs, QBET, EXODUS, and Brain Organoid MAP

 In this talk, I will present smart SANDs (Speedy Analytical Nano-optofluidic Diagnostic systems) to predict and stop the spread of infectious diseases. As an example of smart SANDs, integrated molecular diagnostic systems that comprise three key elements are developed: (1) self-contained sample preparation and liquid biopsy on-chip, (2) ultrafast plasmonic amplification of DNA, RNA, and protein biomarkers, and (3) interface of smartphone optical system. Furthermore, nanoscale SANDs (i.e., nanoplasmonic optical antennas) permit QBET (Quantum Biological Electron Transfer) imaging to detect ET dynamics in mitochondrial cytochrome c during the cell life and death process. The non-invasive real-time QBET spectroscopic imaging of ET in live cells can open a new era in life sciences since it captures spatiotemporal ET dynamics in live cells. Nanoplasmonic optical antennas can also capture the secret communication codes of the bacterial extracellular vesicles (EVs) to better understand the mechanisms of bacterial communication, pathogenesis, and drug resistance.

I will also discuss how human EVs have emerged as a new paradigm in diagnostics and therapeutics, and the recent progress of EV-based applications of EXODUS (EXOsome Detection via the Ultrafast-purification System). Since the EXODUS by harmonic resonator generates high speed, purity, and yield via automated label-free purification of exosomes from various biofluids, it produces the most sensitive detection of liquid biopsy (from blood, tears, urine, or saliva). For example, the significantly improved purification of exosomes from urine samples of cancer patients enables us to obtain efficient multiomics and compare enriched pathways of kidney and bladder cancer. We also developed iTEARS (integrated Tear Exosome Analysis via Rapid-isolation System) to identify biomarkers of proteins and miRNAs and to see the world of diseases in a drop of tears. EXODUS-based iTEARS can provide a solution to identify numerous other diseases, including infectious diseases, neurodegenerative diseases, and cancers.

For personalized precision nanomedicine, I will present the development of brain organoid MAP (Microphysiological Analysis Platforms) with quantum biosensors and therapeutic modulations of mitochondrial electron transport chains. Human-induced pluripotent stem cells-based brain organoid MAP provides an ideal model to address fundamental questions of neuropathogenesis and find solutions for neurodegenerations. In addition, patient-derived brain organoids can recapitulate patient responses and help personalized medicine. Smart SANDs, QBET, EXODUS, and brain organoid MAPs will impact quantitative life sciences and precision nanomedicine via the convergence of biology, chemistry, physics, and engineering.

Hosted by Professor KiBum Lee

~Coffee/tea will be served prior to the lecture~

Location CCB Auditorium (Room 1303)