Structure and Function of Human La-Related Proteins
La-related proteins (LARPs) are a superfamily of RNA-binding proteins whose membership is dictated by the presence of a highly conserved domain called the La motif (LaM). In the vast majority of LARPs, LaM forms the La-module in tandem with a downstream ribonucleotide recognition motif (RRM). Human LARP1 (HsLARP1) is involved in post-transcriptional regulation of certain 5’ terminal oligopyrimidine (5’TOP) mRNAs as well as other mRNAs and several independent studies showed that HsLARP1 is heavily involved in cell proliferation, cell cycle defects, and cancer, where it is significantly upregulated in malignant cells and tissues. Here, we present the structural characterization of the LaM of HsLARP1 as well as the molecular basis for binding of HsLARP1 to the C-terminal domain of poly(A) binding protein (PABP) using solution NMR spectroscopy.
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Hosted by Professor Andy Nieuwkoop