Events Calendar

Download as iCal file
Prof. Giulia Palermo, UC Riverside
Friday, April 23, 2021, 01:00pm - 02:00pm

Giulia Palermo Pic 1Multi-scaling the CRISPR-Cas Revolution from Gene Editing to Viral Detection


In recent years, CRISPR has become synonymous with a transformative genome editing technology that is innovating life science with cutting-edge impact in basic and applied sciences. By enabling the correction of DNA mutations, the CRISPR technology promises to treat a myriad of human genetic diseases, impacting also pharmaceutics, biofuel production, and agriculture with the development of drought-resistant crops. I will report about the use of computational approaches to clarify the molecular basis underlying the genome editing function of CRISPR-Cas9 and newly discovered CRISPR systems that are emerging as powerful tools for viral detection, including the SARS-CoV-2 coronavirus that has posed the current pandemic. We have implemented a multiscale approach, which revealed the mechanistic basis of nucleic acid binding, catalysis, selectivity, and allostery in CRISPR systems. We combined classical molecular dynamics (MD) and enhanced simulation methods to characterize the dynamics of the Cas9 endonuclease and its interplay with nucleic acids over long timescales. Using a Gaussian accelerated MD method, which enables routine access to millisecond timescales, and the Anton-2 supercluster we determined the formation of a catalytically active state, which is prone to DNA cleavages. These computational predictions have been confirmed by single-molecule FRET experiments and by the recent determination through cryo-EM of the activated structure, which remarkably overlaps our model. By applying network models derived from graph theory, we have characterized a mechanism of allosteric regulation, transferring the information of DNA binding to the catalytic sites for cleavages. This mechanism is now being probed in novel Anti-CRISPR proteins, forming multi-mega Dalton complexes with the CRISPR enzyme and used for gene regulation and control. A mixed Quantum Mechanics/Molecular Mechanics (QM/MM) approach is being used to establish the catalytic mechanism of DNA cleavage and the critical role of metal ions, with the ultimate goal of providing information for improving the catalytic efficiency and the specificity of CRISPR-Cas9. Finally, by using multi-microsecond MD simulations we have recently probed a mechanism of DNA-induced of activation in the Cas12a enzyme, which underlies the detection of viral genetic elements, including the SARS-CoV-2 coronavirus. Overall, our outcomes contribute to addressing the mechanistic function of the CRISPR system, providing information that is critical for novel engineering and that could impact the development of improved genome editing tools for biomedical applications.

Hosted by Professor Richard Remsing


For Webex meeting information, please contact Loretta Lupo @ This email address is being protected from spambots. You need JavaScript enabled to view it.