2018 ITC Workshop

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The Calorimetry Conference strives to provide meeting participants with unique outreach opportunities that augment their professional experience. In collaboration with our colleagues at the National Institutes of Health (NIH), we are pleased to co-organize an instructional workshop at The Seventy Third Calorimetry Conference. As an outgrowth of our inaugural workshop on Global and Multi-Method Analysis of Macromolecular Interactions in 2011 and highly successful practical tutorial in 2016, this year's workshop will focus on the design and analysis of Isothermal Titration Calorimetry experiments. The ITC Workshop is scheduled to convene on the Saturday/Sunday (04-05 August 2018) immediately preceding CALCON·ICCT. The instructional staff is comprised of renowned experts who will present lectures in both the ITC Workshop and CALCON·ICCT Scientific Program. Hosting both events at a single location allows us to combine resources and offer an all-inclusive Conference/Workshop Registration Fee of $ 595 and $ 300 for participants and students, respectively. We encourage conferees to avail themselves of this unique opportunity and participate in our jointly organized workshop amidst the scenic splendor of Granlibakken Resort at Lake Tahoe.

Workshop Agenda

The instructional workshop is aimed at researchers whose primary interest is applying biophysical methods to characterize macromolecular interactions and will focus on specific strategies for the design and analysis of isothermal titration calorimetry (ITC) experiments. The workshop is comprised of formal lectures on experimental setup and data analysis accompanied by practical computer exercises in which participants will analyze ITC data employing the SEDPHAT, NITPIC, and GUSSI software platforms. Topics will include the high-precision integration of thermograms, strategies for the global analysis of multiple titration series, and principles/practice of multi-method analyses incorporating ITC and complementary biophysical techniques. The workshop will review analysis of single- and multi-site models spanning the range of cooperative ternary interactions, displacement experiments, and linked binding. A unique feature that sets this workshop apart is the dedicated one-on-one sessions in which each participant is encouraged to analyze his/her ITC data.

Workshop Topics

  • Overview of ITC and experimental design considerations
  • Experimental Strategies: buffer selection and ligand/receptor concentrations
  • Principles of optimization and statistical error analysis
  • Predicting information content with simulation tools
  • Global analysis concepts utilizing SEDPHAT
  • ITC analysis employing NITPIC and GUSSI
  • High-precision integration of ITC thermograms
  • Overview of association models and fitting options
  • Global analysis of multiple titration series
  • Single and multi-site binding models
  • Cooperative ternary interactions
  • Displacement ITC experiments
  • Analysis of linked binding models
  • Principles and practice of multi-method analyses
  • One-on-one practical analysis sessions

Workshop Instructors

  • Dr. Peter W. Schuck, NIBIB National Institutes of Health USA
  • Dr. Huaying Zhao, NIBIB National Institutes of Health USA
  • Prof. Chad Brautigam, Department of Biophysics UT Southwestern Medical Center USA
  • Prof. Sandro Keller, Molecular Biophysics University of Kaiserslautern Germany
  • Dr. Grzegorz Piszczek, NHLBI National Institutes of Health USA