|
Rapid and reversible
changes in cell shape and SA-gal by NAA treatment. (A) BJ cells were
pretreated with 3 mM NAA for
18 days through two subcultures and, at PDL 79, inoculated cells were
treated or untreated with 3 mM NAA for 2 days. At least one positive cell
(stained blue/green) is deliberately included in every photograph to
demonstrate the methodological adequacy of the staining. Changes in cell
shape and SA-gal activity were apparent 2 days after addition or removal
of NAA. (B) Starting at PDL 64, BJ cells underwent six subcultures in the
presence or absence of 3 mM NAA for 54 days and were then inoculated at
PDL 88 and cultivated for 16 days with or without NAA. The ‘young-cell’
phenotypes depended on the presence of NAA but not on pretreatment with
NAA. |
