Is eIF5A an RNA binding protein?
We also compared the binding of eIF-5A and Rev with the 251 nt RRE.
The binding of eIF-5A to 251 nt RRE depends on the presence of deoxyhypusine modification.
Binding to U6
We compared the binding of NS1 and eIf-5A with U6 snRNA.
eIF-5A(dh) binds to U6 but eIF-5A precursor does not.
SELEX search for eIF5A binding sequence
Binding of 6xHis-18K-hyp with random and post-SELEX RNA
Binding of 6xHis-18K-hyp with post-SELEX RNA, U6 RNA, and RRE RNA
The hypusine residue is required for eIF-5A-RNA binding.
The 11 Post-SELEX RNAs share consensus sequence