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Biomolecular NMR - Experiments

  • PFG: Pulsed Field Gradient
  • STE: Stimulated Echo
  • BPP: Bipolar Pulse Pair
  • LED: Longitudinal Eddy Current Delay
  • CC: Convection Correction
  • g: gyromagnetic constant, (26752 for H)
  • D: diffusion rate (m2/s)
  • D for H2O=18.8x10-10 m2/s at 25C
NMR ExperimentBruker Sequences
1H diffusion 1D
Wu (1995) JMRA 115, 260
Jerschow (1997) JMR 125, 372
Chou (2004) JBNMR 29, 299
ledbpgppr2s
Acquires pseudo-2D; F2(1H) - F1QF(diff list)
d20: diffusion delay < 0.2s
gpz6*diff: gradient strength, 0 - 100 % (use <95%)
p30: gradient pulse duration
xau dosy to set up DOSY
Files related;
lists/gp/user/Difframp ( %)
lists/gp/SINE.100 (IntegF)
conf/instr/gradient_calib (G/mm)
expno/difflist (G/cm) = Difframp * IntegF * gradient_calib *10
1H diffusion-15N-HSQC NA
1H diffusion-13C-HSQC hsqcf2stesk
Acquires pseudo-3D; F3(1H) - F2(13C)- F1QF(diff list)
Bruker compatible; d20, p30, gpz6*diff

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