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Biomolecular NMR - Experiments

NMR ExperimentsBruker Sequences
CLEANEX (N15-HSQC) fhsqccxf3gpph3dsk
Acquires Pseudo-3D; F3(HN) - F2(N) - F1(vd list)
Uses vd lists for the exchange time.
If vd=0, a reference spectrum is acquired.
Another reference spectrum with a long d1 is recommended for correction.
Modified from fhsqccxf3gpph
CLEANEX (N15-TROSY) trosycxf3gpphsi19
Not pseudo-3D yet
No reference spectrum yet
SOLEXSY from 15N-NP hsqcf3solexsysk
Acquires Pseudo-3D; F3(HN) - F2(N) - F1(vd list)
Based on Chevelkov et al (2010) JBNMR 46, 227
Modified to use 15N natural polarization instead of HCACONH selection
Requires only 15N-labeling
Uses vd lists for the exchange time.
Odd L0 selects HN + DN and even L0 selects -HN + DN.
Spectra acqured with odd and even L) are subject to add/subtract each other.
Vd list must have duplicate values for L0 selections.

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