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Biomolecular NMR - Experiments

NMR ExperimentsBruker Sequences
15N-HSQC
with PFG coherence selection
hsqcetfpf3gpsi, hsqcetfpf3gpsi2
Acquires 2D; F2(1H) - F1(15N)
Uses PEP (Preservation of equivalent pathways)
Uses Echo and Anti-echo PFG selection
Uses the water flip-back
15N-TROSY
with PFG coherence selection
trosyetfpf3gpsi
Acquires 2D; F2(1H) - F1(15N)
Uses PEP (Preservation of equivalent pathways)
Uses Echo and Anti-echo PFG selection
Uses the water flip-back
15N-HSQC
with Watergate 3919
hsqcf3gpph19
Acquires 2D; F2(1H) - F1(15N)
15N-TROSY
with Watergate 3919
trosyf3gpphsi19.2
Acquires 2D; F2(1H) - F1(15N)
15N-SoFast HMQC sfhmqcf3gpph
Acquires 2D; F2(1H) - F1(15N)
So Fast HMQC by reducing d1
Schanda and Brutscher (2005) JACS 127, 8014
15N-BEST HSQC b_hsqcetf3gpsisk
Acquires 2D; F2(1H) - F1(15N)
Acquires HSQC fast by reducing d1
Schleucher et al (1994) JBNMR 4, 301
Modified from b_hsqcetf3gpsi because of a negative delay.
15N-BEST TROSY b_hsqcetf3gpsisk
Acquires 2D; F2(1H) - F1(15N)
Acquires TROSY fast by reducing d1
Lescop et al (2007) JMR 187, 163
Modified from b_trosyetf3gpsi because of a negative delay.
13C-HSQC hsqcetgp
Acquires 2D; F2(1H) - F1(13C)
Set 13C ppm to 35 ppm (aliphatic) or 125 ppm (aromatic).

Following are various versions.
hsqcetgpsi; sensitivity enhancement version
hsqcetgpsisp2; Uses the C13 shaped inversion pulse
hsqcctetgpsisp; Constant time t1 evolution

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